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English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In SAGE, the ds-cDNA is digested by restriction enzymes (at location ‘X’ and ‘X’+11) to produce 11-nucleotide ‘digitag’ fragments. These digitags are concatenated and sequences using long-read sanger sequencing (different shades of blue indicate digitags from different genes). The sequences are deconvoluted to find the occurrence number of each digitag. Digitag can be used to report on transcription of the gene that the digitag came from is known.[1]
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Ite wetciparik Tipirowe awik otamirowin
Ka ki masihiketc Thomas Shafee
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  1. Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.

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5 Namekosi pisimw 2016

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E tatokonakisitc acitc e tato tipahikaneakMasinasowinicicE iskwakiKa mitatcE iteritaman
mekwatc13 Akokatcic pisimw 2017 à 08:21Masinasowinicic apitc ka meckotcipatakaniwitc 13 Akokatcic pisimw 2017 à 08:21938 × 756 (180 kio)Evolution and evolvabilitytext as paths to avoid artefacts
13 Akokatcic pisimw 2017 à 06:48Masinasowinicic apitc ka meckotcipatakaniwitc 13 Akokatcic pisimw 2017 à 06:48938 × 756 (104 kio)Evolution and evolvabilityupdate sequence text to 'sans-serif' open font for more robust rendering
7 Namekosi pisimw 2016 à 01:27Masinasowinicic apitc ka meckotcipatakaniwitc 7 Namekosi pisimw 2016 à 01:27938 × 756 (104 kio)Evolution and evolvabilityce
7 Namekosi pisimw 2016 à 01:23Masinasowinicic apitc ka meckotcipatakaniwitc 7 Namekosi pisimw 2016 à 01:23938 × 756 (102 kio)Evolution and evolvabilityce
7 Namekosi pisimw 2016 à 01:16Masinasowinicic apitc ka meckotcipatakaniwitc 7 Namekosi pisimw 2016 à 01:16938 × 756 (104 kio)Evolution and evolvabilitydigitag-->tag
5 Namekosi pisimw 2016 à 01:08Masinasowinicic apitc ka meckotcipatakaniwitc 5 Namekosi pisimw 2016 à 01:081 020 × 821 (106 kio)Evolution and evolvabilityUser created page with UploadWizard

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